uid=BNZ,o=lter,dc=ecoinformatics,dc=org all public read knb-lter-bnz.78.10 Roger W. Ruess

Institute of Arctic Biology University of Alaska Fairbanks P.O. Box 757000 Fairbanks AK 99775-0180 United States

(907) 474-7153 (907) 474-6967 rwruess@alaska.edu http://www.iab.uaf.edu/~roger_ruess/ Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 jpdowning@alaska.edu http://www.lter.uaf.edu Mike D Anderson (651) 696-6230 (651) 696-6443 Associated investigator Lee Taylor Assistant Professor

Institute of Arctic Biology 311 Irving I Building University of Alaska Fairbanks Fairbanks AK 99775-7000 USA

(907) 474-6982 (907) 474-7666 Associated investigator 2005-11-17 This study investigated patterns of nitrogen (N) fixation rates, leaf N content, and geographic diversity in the N-fixing bacterium Frankia occurring in symbiosis with Alnus incana ssp. tenuifolia and A. viridis ssp. fruticosa in early and late successional habitats on the Tanana river floodplain and surrounding uplands in the Bonanza Creek Experimental Forest. Frankia diversity was estimated via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the nifD-K spacer region, a non-coding region in the nitrogenase-encoding operon. Specific N-fixation rate was measured with a 15N2 uptake assay and leaf N content via mass spectrometry. Additional parameters measured were soil temperature and moisture, leaf del 15N, and specific leaf area. alder nitrogen fixer actinorhizal alnus frankia Biogeochemistry

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http://www.lter.uaf.edu/data_detail.cfm?datafile_pkey=78 Site information unavailable -148.324901 -148.1435205 64.7666796 64.6719571 2002-06-19 2002-09-01

Completed

Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 jpdowning@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 Bonanza Creek LTER

At each site 10 alder plants were chosen haphazardly; at late succession floodplain sites in which both host species co-occurred 10 plants of each species were chosen. Each site was sampled three times across the growing season, with different plants chosen at each sampling period. Approximately 2.5 grams of nodule tissue was harvested from each plant and assayed for N-fixation rate using a 15N2 uptake assay. Additionally, at each plant soil temperature was measured at 1 cm and 5 cm depths, a soil core was taken for gravimetric moisture measurement, and five leaves were chosen for measurement of specific leaf area, leaf N content, and del 15N. Half of the 15N-assayed nodule sample was selected for DNA extraction and selective amplification of the Frankia nifD-K non-coding spacer locus via polymerase chain reaction (PCR). PCR product was probed for sequence variation by digestion with the restriction enzymes CfoI and HaeIII, followed by high resolution gel electrophoresis. Data were entered and double-checked by Mike Anderson. Soil moisture was calculated as weight of oven-dried soil cores divided by the corresponding fresh weight, times 100. Calculation of specific N-fixation rate (SNF) follows the methods in Anderson et al., 2004. Leaf N content and del 15N were measured via mass spectrometry. Specific leaf area was obtained by dividing dry mass of circular punches taken from leaves by the total area of the punches.

geoReference 2002-06-19 2002-09-01

Once

227_2061_n_fixation_leafn_and_frankia_diversity.txt Restriction fragment patterns of symbiotic Frankia with corresponding nitrogen fixation rate, specific leaf area, leaf N content and del 15N, soil temperature and moisture for each plant sampled 227_2061_n_fixation_leafn_and_frankia_diversity.txt 1 \n \n column \t http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-bnz..&urlTail=/ascii/files/227_2061_n_fixation_leafn_and_frankia_diversity.txt Land Land Land Type string U Upland F Floodplain Stage Stage Successional stage (Late , Early) string Successional stage (Late , Early) Rep Rep Replication; 3 sites/stage/landscape integer Replication; 3 sites/stage/landscape Sampper Sampper Sampling period: 3 per site across season integer Sampling period: 3 per site across season Species Species Host species A. incana or A. viridis string Host species A. incana or A. viridis PLNO PLNO Sequential plant number for given species integer Sequential plant number for given species JULDAY JULDAY Julian Date integer Julian Date 1CM 1CM Soil temperature at 1 cm depth degrees Celsius float celsius real 5CM 5CM Soil temperature at 5 cm depth degrees Celsius float celsius real PERH2O PERH2O Soil percent water by mass proportion float percent real SNF SNF N-fixation rate mol N2 fixed per gram nodule per hr float gram real RF RF Unique restriction fragment pattern float Unique restriction fragment pattern SLA SLA Specific leaf area grams per m^2 float gramsPerSquareMeter real LEAFPERN LEAFPERN Leaf percent N by mass proportion float Leaf percent N by mass proportion DEL DEL Leaf del 15N value float Leaf del 15N value