uid=BNZ,o=EDI,dc=edirepository,dc=org all public read knb-lter-bnz.50.19 Mike Anderson Affiliated Scientist

Olin-Rice Science Center, 115 St. Paul MN 55105 United States

(651) 696-6230 (651) 696-6443 andersonm@macalester.edu http://www.macalester.edu/academics/biology/facultystaff/mikeanderson/ Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Roger Ruess Professor of Biology

Institute of Arctic Biology University of Alaska Fairbanks P.O. Box 757000 Fairbanks AK 99775-0180 United States

(907) 474-7153 (907) 474-6967 Associated party 2005-11-20 This study sought to determine the conversion factor between acetylene reduction and N-fixation rates by root nodules excised from naturally-occurring Alnus tenuifolia and Alnus crispa plants in 18 sites in the BNZ-LTER. N-fixation rate was measured via uptake of 15N2 by excised nodule tissue. Sites were chosen to represent three replicates of early, mid, and late successional habitats on the Tanana River floodplain and in surrounding upland areas. A. tenuifolia was sampled in floodplain sites, and A. crispa in uplands. Parameters measured: acetylene reduction rate, N2 fixation rate, ratio of acetylene reduced to N2 fixed, soil temperature, leaf del 15N, and specific leaf weight. Most of the fixed nitrogen (N) that enters the N cycle in Alaskan forests is fixed biologically by alder (Alnus spp.) in symbiosis with the N-fixing bacterial genus Frankia. Estimates of gross N inputs resulting from this process ultimately rely on accurate measurement of N-fixation by nodule tissue. These rates are typically estimated by measuring rates of acetylene reduction by nodule tissue, then using an empirically determined conversion factor to obtain the corresponding N fixation rate. The purposes of this study were to determine: 1) the conversion factor(s) for Alnus tenuifolia and Alnus crispa in the Bonanza Creek Experimental Forest, and 2) whether the conversion factor varied between plants occurring in different successional habitats. acetylene reduction nitrogen-15 Alnus tenuifolia Alnus crispa alder nitrogen fixer actinorhizal plants alnus frankia plants bacteria Biogeochemistry

This work has been produced as part of the Long Term Ecological Research Program and data users should adhere to the Data Use Agreement of the Long Term Ecological Research Network. The consumer of these data (“Data User” herein) has an ethical obligation to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or coauthorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available “as is.” The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User should properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator(s), Year of Data Publication, Title of Dataset, Publisher, Dataset identifier, Dataset URL, Dataset DOI. For Example: Van Cleve, Keith; Chapin, F. Stuart; Ruess, Roger W. 2016. Bonanza Creek Experimental Forest: Hourly Temperature (sample, min, max) at 50 cm and 150 cm from 1988 to Present, Bonanza Creek LTER - University of Alaska Fairbanks. BNZ:1, http://www.lter.uaf.edu/data/data-detail/id/1. doi:10.6073/pasta/725db90d86686be13e6d6b2da5d61217.

The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example: Data are provided by the Bonanza Creek LTER, a partnership between the University of Alaska Fairbanks, and the U.S. Forest Service. Significant funding for collection of these data was provided by the National Science Foundation Long-Term Ecological Research program (NSF Grant numbers DEB-2224776, DEB-1636476, DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629) and by the USDA Forest Service, Pacific Northwest Research Station (Agreement # RJVA-PNW-20-JV-11261932-018).

The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed.

The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available in its present condition. The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

http://www.lter.uaf.edu/data/data-detail/id/50 Dataset geographic description information is unavailable. {datafile/geoCoverage is empty} -148.3790685 -148.1435205 64.7666796 64.66966 2001-06-25 2001-08-10

Completed

Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 Bonanza Creek LTER

At each site 10 alder plants were chosen haphazardly. Approximately 5 grams of nodule tissue was harvested from each plant, half of which was exposed to acetylene for 2.5 minutes, and half to 15N2 for 10 minutes. A sample of the 15N2 composition of the incubation atmosphere was also taken. Additionally, at each plant soil temperature was measured at a depth corresponding to that at which most nodules occurred, and five leaves were chosen for measurement of specific leaf weight and del 15N. Ethylene production resulting from acetylene reduction by nodules was quantified via gas chromatography. Nodule 15N2 uptake, incubation atmosphere composition, and leaf del 15N were measured via mass spectrometry. Data were entered and double-checked by Mike Anderson. ARA conversion factors for each plant were calculated by dividing the acetylene reduction rate by the rate of N-fixation calculated from 15N2 uptake. 15N2 uptake was converted to an estimate of N-fixation rate by correcting for the 15N2 composition of the incubation atmosphere. Specific leaf weight was obtained by dividing the total area of circular punches taken from leaves by the dry mass of the punches. Leaf del 15N was obtained via mass spectrometry.

FP1A -- FP1A is located on a low early successional terrace (1.8 - 2 m above winter low river level) adjacent to the Tanana River. Vegetation establishment occurred in 1982 to 1983. Significant bank erosion has occurred since the establishment of the study area, resulting in loss of some study plots.. Site alternate names: 211, GPIIC, Gravel Pit Willow, BCII, IIIAC, IIIAT, IIIBC, IIIBT -148.2576912 -148.2576912 64.69859835 64.69859835 FP1B -- FP1B is located on a low early successional terrace adjacent to the Tanana River.. Site alternate names: 212 -148.149437 -148.149437 64.73146502 64.73146502 FP1C -- FP1C is located on a low early successional terrace adjacent to the Tanana River.. Site alternate names: 213 -148.2744455 -148.2744455 64.68671338 64.68671338 BP1 -- Beneath the overstory of balsam poplar in these forests (approximately 100 years of age) grows a relatively dense understory of thin-leaf alder, with rose (Rosa acicularis) and high-bush cranberry (Viburnum edule) present within the shrub layer, and a scattered ground cover of non-vascular plants, graminoids, and forbs (Ruess et al. 2013 in press). . Site alternate names: -148.241 -148.241 64.67066 64.67066 BP2 -- Beneath the overstory of balsam poplar in these forests (approximately 100 years of age) grows a relatively dense understory of thin-leaf alder, with rose (Rosa acicularis) and high-bush cranberry (Viburnum edule) present within the shrub layer, and a scattered ground cover of non-vascular plants, graminoids, and forbs (Ruess et al. 2013 in press). . Site alternate names: -148.24783 -148.24783 64.66966 64.66966 BP3 -- Beneath the overstory of balsam poplar in these forests (approximately 100 years of age) grows a relatively dense understory of thin-leaf alder, with rose (Rosa acicularis) and high-bush cranberry (Viburnum edule) present within the shrub layer, and a scattered ground cover of non-vascular plants, graminoids, and forbs (Ruess et al. 2013 in press). . Site alternate names: -148.32683 -148.32683 64.67083 64.67083 FP4A -- Mature white spruce. Site alternate names: 241, SMVIIIC, FSL-LD-FP4A_ws, VIIIWS, VIIIAC -148.2368605 -148.2368605 64.67878586 64.67878586 FP4B -- Mature white spruce. Site alternate names: 242, FSL-LD-FP4B_ws, VIIIYI, VIIIBC -148.2707843 -148.2707843 64.67129926 64.67129926 FP4C -- Mature white spruce. Site alternate names: 243, LV132, FSL-LD-FP4C_ws -148.1827217 -148.1827217 64.71354433 64.71354433 UP2A -- Closed Mixed White Spruce - Paper Birch Stand. Site alternate names: 121, LTER #121, FSL-LD-UP2A_birch -148.35568 -148.35568 64.69546067 64.69546067 UP2B -- Closed Mixed White Spruce - Paper Birch Stand. Site alternate names: 122, LTER #122, FSL-LD-UP2B_birch -148.3641664 -148.3641664 64.6905617 64.6905617 UP2C -- Closed Mixed Aspen - White Spruce Stand. Site alternate names: 123, FSL-LD-UP2C_aspen -148.3834237 -148.3834237 64.68927697 64.68927697 UP3A -- Mature white spruce spruce stand on a south-facing slope. Site alternate names: 131, LTER #131, Tower site: Parks Hwy, South side: Gregory stand #148, Zasada stand: Bonanza Creek - 1 (seed studies), Semi-intensive site #12, Juday, Parks Loop West, Tower Site, FSL-LD-UP3A_ws -148.2795018 -148.2795018 64.76742521 64.76742521 UP3B -- Closed White Spruce Forest. Site alternate names: 132, LTER #132, FSL-LD-UP3B_ws -148.2508148 -148.2508148 64.76051099 64.76051099 UP3C -- Closed White Spruce Forest. Site alternate names: 133, LTER #133, FSL-LD-UP3C_ws -148.3306337 -148.3306337 64.72538077 64.72538077 2001-06-25 2001-08-10

Daily, 1 site (10 plants) per day

Keith Van Cleve Bonanza Creek LTER Lead Principal Investigator Principal Investigator F.S. Stuart Chapin Bonanza Creek LTER Lead Principal Investigator

Department of Biology and Wildlife; Institute of Arctic Biology: University of Alaska Fairbanks 193 Arctic Health P.O. Box 757000 Fairbanks AK 99775 United States

(907) 455-6408 (907) 474-6967 terry.chapin@alaska.edu http://terrychapin.org/ Principal Investigator Roger W. Ruess Bonanza Creek LTER Lead Principal Investigator

Institute of Arctic Biology University of Alaska Fairbanks P.O. Box 757000 Fairbanks AK 99775-0180 United States

(907) 474-7153 (907) 474-6967 rwruess@alaska.edu http://www.iab.uaf.edu/~roger_ruess/ Principal Investigator Michelle Cailin Mack Bonanza Creek LTER Lead Principal Investigator

Northern Arizona University: Ecosystem Science and Society Center (ECOSS) PO Box 5620 Flagstaff AZ 86011 United States

(928) 523-9415 Michelle.Mack@nau.edu https://macklab.nau.edu/ Principal Investigator NSF Grant numbers DEB-2224776, DEB-1636476, DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629 USDA Forest Service, Pacific Northwest Research Station (Agreement # RJVA-PNW-20-JV-11261932-018) 226_2060_ARA15N2_ratios.txt Acetylene reduction values, 15N2 uptake rates, leaf del 15N, and specific leaf weights from Alnus tenuifolia in early, mid, and late succession on the Tanana floodplain and A. crispa in early, mid, and late succession in the surrounding uplands. 226_2060_ARA15N2_ratios.txt 2 \n \n column \t http://www.lter.uaf.edu/php/download_data.php?f=/ascii/files/226_2060_ARA15N2_ratios.txt Land Land Upland or Floodplain site string UP Upland FP Floodplain Species Species Species Code string AC A. crispa AT A. tenuifolia Stage Stage Successional stage string Successional stage Repsite Repsite Replicate site; 3 sites/stage/landscape integer Replicate site; 3 sites/stage/landscape PLNO PLNO Plant number, 1-90 for each species integer Plant number, 1-90 for each species JULDAY JULDAY Julian day of sampling integer ddd real Soiltemp Soiltemp Soil temperature at sample degrees Celsius float celsius real ARA ARA Acetylene reduction rate mol float Moles real PERNODN PERNODN Nodule percent nitrogen by mass proportion float dimensionless real N2FIX N2FIX Nitrogen fixation rate from 15N uptake assay mol N2 fixed per gram nodule per hr float gramnoduleperhr real Ratio Ratio RATIO of Acetylene reduction rate/Nitrogen fixation rate float dimensionless real DEL15N DEL15N Leaf del 15N value float dimensionless real NA SLW NA SLW Specific leaf weight cm^2 per gram float cm^2pergram real