uid=BNZ,o=EDI,dc=edirepository,dc=org all public read knb-lter-bnz.326.20 Merritt R Turetsky Senior Investigator

Institute of Arctic and Alpine Research, University of Colorado-Boulder Boulder CO 80309 United States

merritt.turetsky@colorado.edu Evan Kane Affiliated Scientist

School of Forest Resources and Environmental Science, Michigan Technological University Houghton MI 49931 United States

(906) 482-6303 eskane@mtu.edu Catherine M Dieleman Post-doctoral Research Associate

Department of Integrative Biology, University of Guelph 50 Stone Road East Guelph Ontario N1G2W1 Canada

226-984-9461 cdielema@uoguelph.ca Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Danielle Rupp Ph.D Student

School of Forest Resources and Environmental Science (SFRES), Michigan Technological University Houghton Michigan 49931 United States

(608) 636-3306 Associated party Molly Conlin

166 Plant Biology Lab East Lansing Michigan 48824 United States

(517)-353-2953 Associated party Sara Klapstein M.S. Candidate

Integrative Biology, Science Complex Rm 2408, University of Guelph Guelph Ontario N1G 2W5 Canada

Associated party Claire C Treat Research Technician

Dept. Plant Biology; Michigan State University East Lansing Michigan 48824 United States

517-353-2953 517-353-1926 Associated party 2019-11-29 Methane fluxes were collected at APEX rich fen during the summers of 2005 - 2011 and 2015-2016. APEX Fen is located in the Bonanza Creek LTER outside of Fairbanks, AK. APEX Fen uses a full-factorial water table (flooded or raised, lowered or drained, and control treatments, as well as a moisture gradient). A warming manipulation (warmed, unwarmed treatment) was in place in 2005-2011, but ceased by 2014. In 2015, vegetation manipulation subplots were installed within the water table treatments. This new experiment was called the Next Generation Experiment peatland experimental warming wetlands soil climate manipulation water table manipulation methane Biogeochemistry

This work has been produced as part of the Long Term Ecological Research Program and data users should adhere to the Data Use Agreement of the Long Term Ecological Research Network. The consumer of these data (“Data User” herein) has an ethical obligation to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or coauthorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available “as is.” The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User should properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator(s), Year of Data Publication, Title of Dataset, Publisher, Dataset identifier, Dataset URL, Dataset DOI. For Example: Van Cleve, Keith; Chapin, F. Stuart; Ruess, Roger W. 2016. Bonanza Creek Experimental Forest: Hourly Temperature (sample, min, max) at 50 cm and 150 cm from 1988 to Present, Bonanza Creek LTER - University of Alaska Fairbanks. BNZ:1, http://www.lter.uaf.edu/data/data-detail/id/1. doi:10.6073/pasta/725db90d86686be13e6d6b2da5d61217.

The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example: Data are provided by the Bonanza Creek LTER, a partnership between the University of Alaska Fairbanks, and the U.S. Forest Service. Significant funding for collection of these data was provided by the National Science Foundation Long-Term Ecological Research program (NSF Grant numbers DEB-2224776, DEB-1636476, DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629) and by the USDA Forest Service, Pacific Northwest Research Station (Agreement # RJVA-PNW-20-JV-11261932-018).

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The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available in its present condition. The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

http://www.lter.uaf.edu/data/data-detail/id/326 Dataset geographic description information is unavailable. {datafile/geoCoverage is empty} -148.31034 -148.31034 64.70138 64.70138 2005-06-16 2019-07-31

Gas fluxes not near zero that did not show a trend (9%) were discarded.

APEXCon_Schematic.pdf which is already on the website

Ongoing

Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 Bonanza Creek LTER

APEX Fen uses a full-factorial water table (flooded or raised, lowered or drained, and control treatments, as well as a moisture gradient in 2005-2011) by warming manipulation (warmed, unwarmed treatment) in 2005-2011. Water table treatments were randomly assigned to each plot. Permanent gas measurement collars were established within these plots and randomly assigned to a warming treatment, which had ceased by 2014. The Next Generation experiment was initiated in 2015 by E.Kane, installing a series of new plots in all three water table treatments (i.e. Control 7- 14 + NP, Lowered 5-12, NP, Raised 1, 4, 7, 8 NP). Plots Lowered 5,6 and Raised 1, 4 were repurposed into the Next Gen. Plots Control 1-6, Lowered 1-4, and Raised 2, 3, 5, 6 all remained under their original treatments in the Baseline Experiment. The Next Generation experiment involves a combination of 'sedge removal' or 'all other species removal' treatments across the three water table treatments.

FIELD: Data were collected from 18 collars located within the water table (by warming treatments in 2005-2011) at APEX Fen as described above. In 2005-2011, moisuture gradient collars range from a black spruce site (Gradient 1), a site dominated by willow-birch shrubs (Gradient 2), a tussock site (Gradient 3, located on a tussock), and a site dominated by equisetum and carex (Gradient 4). Moisture gradient measurements were discontinued by 2014, whereas the 18 collars within the water table treatments continued to be monitored in 2015. Four collars, two in the lowered plot and two in the raised plot, were not monitored in 2016, as the four were part of the Next Generation experiment installed at the end of the 2015 field season. The Next Generation experiment plots was allowed to "settle" without flux measurements during the summer of 2016. In 2019 soil collars were installed in at all Next Generation plots in May. Starting in June both the Baseline Experiment (i.e. Control 1-6, Lowered 1-4, and Raised 2, 3, 5, 6) and the Next Generation Experiment (i.e. Control 7- 14 + NP, Lowered 5-12, NP, Raised 1, 4, 7, 8 NP) were monitored. Methane samples were collected weekly to bi-weekly from clear static chambers (60 cm x 60 cm x 60 cm) placed on collars that are permanently embedded in the peat. Collars were sealed using foam tape. LAB: Gas samples were collected into 20 mL syringes over a period of 30 -40 minutes and analyzed for methane concentrations using a gas chromatograph equipped with a flame ionization detector (FID). Gas fluxes not near zero that did not show a trend (9%) were discarded.

apexcon -- lowland open fen with daily access during the growing season. Site alternate names: control, Fen, Fencon, BC_FEN -148.3138278 -148.3138278 64.70129167 64.70129167 apexele -- lowland open fen with daily access during the growing season. Site alternate names: raised, Fen, Fenrai -148.31034 -148.31034 64.70138 64.70138 apexlow -- lowland open fen with daily access during the growing season. Site alternate names: lowered, Fen, Fenlow -148.31034 -148.31034 64.70138 64.70138 BZBS -- A well drained black spruce stand. A well drained black spruce stand.. Site alternate names: BS -148.31132 -148.31132 64.70082 64.70082 BZWB -- A site dominated by shrubs, including willow and birch species.. Site alternate names: WB -148.31132 -148.31132 64.70082 64.70082 BZTG -- A tussock site dominated by Calamagrastis spp. with a sparse covering of Drepanocladus.. Site alternate names: TG -148.31132 -148.31132 64.70082 64.70082 BZEC -- An intermediate rich fen, which includes a diverse groundlayer mixture of Sphagnum, brown mosses, and Drepanocladus spp., and a sparse cover of Equisetum. . Site alternate names: EC -148.31132 -148.31132 64.70082 64.70082 2005-06-16 2019-07-31

Weekly

Keith Van Cleve Bonanza Creek LTER Lead Principal Investigator Principal Investigator F.S. Stuart Chapin Bonanza Creek LTER Lead Principal Investigator

Department of Biology and Wildlife; Institute of Arctic Biology: University of Alaska Fairbanks 193 Arctic Health P.O. Box 757000 Fairbanks AK 99775 United States

(907) 455-6408 (907) 474-6967 terry.chapin@alaska.edu http://terrychapin.org/ Principal Investigator Roger W. Ruess Bonanza Creek LTER Lead Principal Investigator

Institute of Arctic Biology University of Alaska Fairbanks P.O. Box 757000 Fairbanks AK 99775-0180 United States

(907) 474-7153 (907) 474-6967 rwruess@alaska.edu http://www.iab.uaf.edu/~roger_ruess/ Principal Investigator Michelle Cailin Mack Bonanza Creek LTER Lead Principal Investigator

Northern Arizona University: Ecosystem Science and Society Center (ECOSS) PO Box 5620 Flagstaff AZ 86011 United States

(928) 523-9415 Michelle.Mack@nau.edu https://macklab.nau.edu/ Principal Investigator NSF Grant numbers DEB-2224776, DEB-1636476, DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629 USDA Forest Service, Pacific Northwest Research Station (Agreement # RJVA-PNW-20-JV-11261932-018) 326_APEX_fen_methane_flux_2005-2011.txt Methane fluxes from an Alaskan rich fen 326_APEX_fen_methane_flux_2005-2011.txt 1 \n \n column \t http://www.lter.uaf.edu/php/download_data.php?f=/ascii/files/326_APEX_fen_methane_flux_2005-2011.txt year year Year of sampling integer dimensionless real date date date of sampling (yyyy-mm-dd) string yyyy-mm-dd JD JD Day of year of sampling (DOY) integer dimensionless real hour hour time of sampling, rounded to nearest hour integer dimensionless real Plot Plot water table manipulation plot string Control control treatment Lowered lowered or drained treatment Raised raised or flooded treatment Gradient gradient or moisture gradient collar collar permanent collar embedded in peat, identifier of measurement location integer permanent collar embedded in peat, identifier of measurement location warm warm whether the collar was assigned to warming treatment with an open-topped chamber string yes warmed no unwarmed CH4 flux CH4 flux Volumetric methane flux float mgCH4/m2/d real -1000.000 10000.000 r2 r2 R2 value float dimensionless real 326_APEX_fen_methane_flux_2015_2016.txt Static methane flux measurements at APEX alpha 2015 and 2016 field seasons 326_APEX_fen_methane_flux_2015_2016.txt 1 \n \n column \t http://www.lter.uaf.edu/php/download_data.php?f=/ascii/files/326_APEX_fen_methane_flux_2015_2016.txt year year Year of sampling integer dimensionless real date date date of sampling (yyyy-mm-dd) string yyyy-mm-dd JD JD Day of year of sampling (DOY) integer dimensionless real hour hour time of sampling, rounded to nearest hour integer dimensionless real Plot Plot water table manipulation plot string Control control treatment Lowered lowered or drained treatment Raised raised or flooded treatment Gradient gradient or moisture gradient collar collar permanent collar embedded in peat, identifier of measurement location integer permanent collar embedded in peat, identifier of measurement location warm warm whether the collar was assigned to warming treatment with an open-topped chamber string yes warmed no unwarmed CH4 flux CH4 flux Volumetric methane flux float mgCH4/m2/d real -1000.000 10000.000 r2 r2 R2 value float dimensionless real 326_APEX_fen_methane_flux_2019.txt This dataset contains the weekly measurements of CH4 flux 326_APEX_fen_methane_flux_2019.txt 1 \n \n column \t http://www.lter.uaf.edu/php/download_data.php?f=/ascii/files/326_APEX_fen_methane_flux_2019.txt year year Year of sampling integer dimensionless real date date date of sampling (yyyy-mm-dd) string yyyy-mm-dd JD JD Day of year of sampling (DOY) integer dimensionless real hour hour time of sampling, rounded to nearest hour integer dimensionless real Plot Plot water table manipulation plot string Control control treatment Lowered lowered or drained treatment Raised raised or flooded treatment Gradient gradient or moisture gradient collar collar permanent collar embedded in peat, identifier of measurement location integer permanent collar embedded in peat, identifier of measurement location warm warm whether the collar was assigned to warming treatment with an open-topped chamber string yes warmed no unwarmed CH4 flux CH4 flux Volumetric methane flux float mgCH4/m2/d real -1000.000 10000.000 r2 r2 R2 value float dimensionless real APEXCon_Schematic.pdf Site schematic for APEX mainulation and transect plots. APEXCon_Schematic.pdf pdf http://www.lter.uaf.edu/php/download_data.php?f=/ascii/supplementaryFiles/APEXCon_Schematic.pdf pdf