uid=BNZ,o=EDI,dc=edirepository,dc=org all public read knb-lter-bnz.229.21 Anthony David McGuire Co-Principal Investigator

Alaska Cooperative Fish and Wildlife Research Unit University of Alaska Fairbanks Irving I Building Fairbanks AK 99775 United States

(907) 474-6242 (907) 474-7872 admcguire@alaska.edu http://people.iab.uaf.edu/dave_mcguire Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Joy Clein Research Professional

Institute of Arctic Biology University of Alaska Fairbanks P.O. Box 757000 Fairbanks AK 99775-7000 United States

(907) 474-5660 (907) 474-6967 Associated party 2007-01-01 We collected black spruce needle from several sites located near Fairbanks Ak, and Delta, Ak in August 1999 (Sites designated here as S1, S2, and S3) were located in Fairbanks (64o40?N, 148o15?W), Alaska (S1: 64o52.164? N, 147o51.462? W; S2: 64o52.058? N, 147o51.378? W; S3: 64o51.603? N, 147o52.789? W) with 2 additional sites (S4, S5) in Delta, Alaska (64010? N, 145030? W). In each stand, 30 trees were randomly selected for sampling. Within each tree, five shoots were collected from the southern aspect of the mid-canopy height from each of the following age classes: 0-, 1-, 4-, 9-, and 19-years old. In two of the Fairbanks stands (S2, S3), 19-year old needles were not present. A total of 690 samples over the five stands include 150 samples per stand in three stands (S1, S4, S5) and 120 samples per stand in the other two stands (S2, S3). Needles were returned to the lab and nitrogen content was determined. We used a three-factor nested analysis of variance (ANOVA) to evaluate differences in needle N concentration among the ages of needles on a tree, among trees nested within a stand, and among stands. Ages and stands were treated as fixed factors and tree was treated as a random factor. The ANOVA and means testing for these differences used Proc GLM in the SAS statistical package with needle N content as the dependent variable and needle age, tree, and stand as independent variables. The GLM procedure handled the problem of missing treatment combinations within the ANOVA. Type III sum of squares was used to test the effects of factors without interactions black spruce Fairbanks nitrogen Biogeochemistry

This work has been produced as part of the Long Term Ecological Research Program and data users should adhere to the Data Use Agreement of the Long Term Ecological Research Network. The consumer of these data (“Data User” herein) has an ethical obligation to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or coauthorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available “as is.” The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User should properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator(s), Year of Data Publication, Title of Dataset, Publisher, Dataset identifier, Dataset URL, Dataset DOI. For Example: Van Cleve, Keith; Chapin, F. Stuart; Ruess, Roger W. 2016. Bonanza Creek Experimental Forest: Hourly Temperature (sample, min, max) at 50 cm and 150 cm from 1988 to Present, Bonanza Creek LTER - University of Alaska Fairbanks. BNZ:1, http://www.lter.uaf.edu/data/data-detail/id/1. doi:10.6073/pasta/725db90d86686be13e6d6b2da5d61217.

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http://www.lter.uaf.edu/data/data-detail/id/229 Dataset geographic description information is unavailable. {datafile/geoCoverage is empty} -147.8798 -145.5 64.8694 64.1667 1999-06-01 1999-09-01

Completed

Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 Bonanza Creek LTER

Field methods: Samples were collected from all 5 sites. In each stand, 30 trees were randomly selected for sampling. Within each tree, five shoots were collected from the southern aspect of the mid-canopy height from each of the following age classes: 0-, 1-, 4-, 9-, and 19-years old. In two of the Fairbanks stands (S2, S3), 19-year old needles were not present. A total of 690 samples over the five stands include 150 samples per stand in three stands (S1, S4, S5) and 120 samples per stand in the other two stands (S2, S3). Lab methods: Samples were oven dried at 50 degC for three to five days, and needles were removed from shoots, weighed, ground through a 20 um mesh screen, and analyzed for total N on a LECO 2000 CNS Autoanalyzer (St. Joseph, Michigan). We used a three-factor nested analysis of variance (ANOVA) to evaluate differences in needle N concentration among the ages of needles on a tree, among trees nested within a stand, and among stands. Ages and stands were treated as fixed factors and tree was treated as a random factor. The ANOVA and means testing for these differences used Proc GLM in the SAS statistical package with needle N content as the dependent variable and needle age, tree, and stand as independent variables. The GLM procedure handled the problem of missing treatment combinations within the ANOVA. Type III sum of squares was used to test the effects of factors without interactions.

1999-06-01 1999-09-01

once

Keith Van Cleve Bonanza Creek LTER Lead Principal Investigator Principal Investigator F.S. Stuart Chapin Bonanza Creek LTER Lead Principal Investigator

Department of Biology and Wildlife; Institute of Arctic Biology: University of Alaska Fairbanks 193 Arctic Health P.O. Box 757000 Fairbanks AK 99775 United States

(907) 455-6408 (907) 474-6967 terry.chapin@alaska.edu http://terrychapin.org/ Principal Investigator Roger W. Ruess Bonanza Creek LTER Lead Principal Investigator

Institute of Arctic Biology University of Alaska Fairbanks P.O. Box 757000 Fairbanks AK 99775-0180 United States

(907) 474-7153 (907) 474-6967 rwruess@alaska.edu http://www.iab.uaf.edu/~roger_ruess/ Principal Investigator Michelle Cailin Mack Bonanza Creek LTER Lead Principal Investigator

Northern Arizona University: Ecosystem Science and Society Center (ECOSS) PO Box 5620 Flagstaff AZ 86011 United States

(928) 523-9415 Michelle.Mack@nau.edu https://macklab.nau.edu/ Principal Investigator NSF Grant numbers DEB-2224776, DEB-1636476, DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629 USDA Forest Service, Pacific Northwest Research Station (Agreement # RJVA-PNW-20-JV-11261932-018) 5standsN.txt Black spruce needles were collected and analyzed for nitrogen content. 5standsN.txt 1 \n \n column , http://www.lter.uaf.edu/php/download_data.php?f=/ascii/files/5standsN.txt Stand Stand Stand integer Stand needleage needleage needleage integer needleage Tree# Tree# Tree# integer Tree# N(%) N(%) N(%) float dimensionless real