uid=BNZ,o=EDI,dc=edirepository,dc=org all public read knb-lter-bnz.189.19 Isla Myers-Smith

405 CW Biological Sciences Building, Dept. Biological Sciences University of Alberta, , AB, T6G 2E9 Edmonton Aberta T6G 2E9 Canada

(780) 492-1295 myerssmi@ualberta.ca http://www.ualberta.ca/~myerssmi/home.html Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu F Stuart Chapin Professor of Ecology

Institute of Arctic Biology: University of Alaska Fairbanks Fairbanks AK 99775 United States

(907) 455-6408 (907) 474-6967 Associated party Jennifer Harden USGS, Adjunct Professor of Soil Science, University of Alaska Fairbanks

U.S. Geological Survey 345 Middlefield Road, MS 962 Menlo Park CA 94025 United States

(650) 329-4949 (650) 329-4920 Associated party Anthony David McGuire Assistant Professor of Landscape Ecology

Alaska Cooperative Fish and Wildlife Research Unit University of Alaska Fairbanks Irving I Building Fairbanks AK 99775 United States

(907) 474-6242 (907) 474-7872 Associated party 2005-11-12 This data set contains diatom assemblages for 3 cores from the center, moat and surrounding burn (0, 12, and 30 m) of the BBC collapse scar. Three well-preserved cores were collected in March 2003 with a gasoline-powered permafrost corer. Cores were stored frozen and sampled using a radial saw. The cores were sampled every two cm for macrofossil, diatom analysis and chemistry. Diatoms have been shown to be a useful indicator of peatland succession, local hydrology, and fire disturbance (Kienel et al., 1999, Moser et al., 2000, R�hland et al., 2000). Diatoms are more sensitive to changes in water levels than the commonly used peat indicators such as pollen and macrofossil analysis (R�hland et al., 2000). In this study, we employed diatom analysis to indicate shifts in water chemistry and to ascertain the response of this ecosystem to disturbance. To survey the diatom community, we processed three cores sampled every 2 cm by depth from the bog, moat and burn using methods described by the Paleoecological Environmental Assessment and Research Laboratory (PEARL) at Queen�s University and personal communications (Kathleen R�hland and John P. Smol). We digested the dried material, consisting of organic matter with varying amounts of sediment, in Kjeldahl digestion tubes in a heating block. We used 50:50 solution by molecular weight of concentrated H2SO4 and HNO3 and digested for 3 days at 95?C or until the disappearance of all organic matter. We diluted the resulting solution with deionized water to a neutral pH. We mounted the samples using the Pleurax high refractive index mounting medium (prepared by W. Dailey, University of Pennsylvania). To determine the prevalence of the different diatoms, we counted 400 valves (or for one sparse sample, four slides) for each sample. We identified samples according to Foged (1981), Krammer and Lange-Bertalot (1991) and personal communications with K. R�hland. We measured bulk density, %C and %N for all soil samples. We oven-dried at 50 - 65�C and ground all samples before analysis. We analyzed samples for %C and %N using a Carlo Erba EA1108 CHNS analyzer (CE Instruments, Milan, Italy) and a COSTECH ECS 4010 CHNS-O analyzer (Costech Analytical Technologies Inc., Valencia, CA,USA). Sample standard errors were � 0.01% for nitrogen, � 0.45% for carbon. To indicate fire events in the surrounding ecosystem, charcoal layers in the cores were quantified. We estimated charcoal by emptying dried samples of a known volume and depth (on mean 4.5 cm3) over a 10 cm x 10 cm grid and counting macroscopic charcoal fragments (greater than 0.05 mm in diameter) in each cm grid cell. moat diatoms fires macrofossil bogs density carbon nitrogen soil samples charcoal Biogeochemistry

This work has been produced as part of the Long Term Ecological Research Program and data users should adhere to the Data Use Agreement of the Long Term Ecological Research Network. The consumer of these data (“Data User” herein) has an ethical obligation to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or coauthorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available “as is.” The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User should properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator(s), Year of Data Publication, Title of Dataset, Publisher, Dataset identifier, Dataset URL, Dataset DOI. For Example: Van Cleve, Keith; Chapin, F. Stuart; Ruess, Roger W. 2016. Bonanza Creek Experimental Forest: Hourly Temperature (sample, min, max) at 50 cm and 150 cm from 1988 to Present, Bonanza Creek LTER - University of Alaska Fairbanks. BNZ:1, http://www.lter.uaf.edu/data/data-detail/id/1. doi:10.6073/pasta/725db90d86686be13e6d6b2da5d61217.

The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example: Data are provided by the Bonanza Creek LTER, a partnership between the University of Alaska Fairbanks, and the U.S. Forest Service. Significant funding for collection of these data was provided by the National Science Foundation Long-Term Ecological Research program (NSF Grant numbers DEB-2224776, DEB-1636476, DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629) and by the USDA Forest Service, Pacific Northwest Research Station (Agreement # RJVA-PNW-20-JV-11261932-018).

The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed.

The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available in its present condition. The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

http://www.lter.uaf.edu/data/data-detail/id/189 Dataset geographic description information is unavailable. {datafile/geoCoverage is empty} -148.3334833 -148.3334833 64.6408 64.6408 2003-03-01 2003-03-29

Completed

Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 uaf-bnz-im-team@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 Bonanza Creek LTER

This data set contains diatom assemblages for 3 cores from the center, moat and surrounding burn (0, 12, and 30 m) of the BBC collapse scar and associated soil characteristics.

Field methods: Three well-preserved cores were collected in March 2003 with a gasoline-powered permafrost corer. Lab methods: Cores were stored frozen and sampled using a radial saw. The cores were sampled every two cm for macrofossil, diatom analysis and chemistry. To survey the diatom community, we processed three cores sampled every 2 cm by depth from the bog, moat and burn using methods described by the Paleoecological Environmental Assessment and Research Laboratory (PEARL) at Queen�s University and personal communications (Kathleen R�hland and John P. Smol). We digested the dried material, consisting of organic matter with varying amounts of sediment, in Kjeldahl digestion tubes in a heating block. We used 50:50 solution by molecular weight of concentrated H2SO4 and HNO3 and digested for 3 days at 95?C or until the disappearance of all organic matter. We diluted the resulting solution with deionized water to a neutral pH. We mounted the samples using the Pleurax high refractive index mounting medium (prepared by W. Dailey, University of Pennsylvania). To determine the prevalence of the different diatoms, we counted 400 valves (or for one sparse sample, four slides) for each sample. We identified samples according to Foged (1981), Krammer and Lange-Bertalot (1991) and personal communications with K. R�hland. We measured bulk density, %C and %N for all soil samples. We oven-dried at 50 - 65�C and ground all samples before analysis. We analyzed samples for %C and %N using a Carlo Erba EA1108 CHNS analyzer (CE Instruments, Milan, Italy) and a COSTECH ECS 4010 CHNS-O analyzer (Costech Analytical Technologies Inc., Valencia, CA,USA). Sample standard errors were � 0.01% for nitrogen, � 0.45% for carbon. To indicate fire events in the surrounding ecosystem, charcoal layers in the cores were quantified. We estimated charcoal by emptying dried samples of a known volume and depth (on mean 4.5 cm3) over a 10 cm x 10 cm grid and counting macroscopic charcoal fragments (greater than 0.05 mm in diameter) in each cm grid cell.

BBC collapse scar -- The BBC collapse scar is located in Interior Alaska, USA, adjacent to the Bonanza Creek LTER on the south side of the Tanana River, approximately half way between Fairbanks and Nenana. The scar is located 0.5 km south of the river bank in a low lying black spruce tussock dominated wetland that was burned in June-July 2001 by the Survey Line fire. . Site alternate names: -148.3334833 -148.3334833 64.6408 64.6408 2003-03-01 2003-03-29

once

Keith Van Cleve Bonanza Creek LTER Lead Principal Investigator Principal Investigator F.S. Stuart Chapin Bonanza Creek LTER Lead Principal Investigator

Department of Biology and Wildlife; Institute of Arctic Biology: University of Alaska Fairbanks 193 Arctic Health P.O. Box 757000 Fairbanks AK 99775 United States

(907) 455-6408 (907) 474-6967 terry.chapin@alaska.edu http://terrychapin.org/ Principal Investigator Roger W. Ruess Bonanza Creek LTER Lead Principal Investigator

Institute of Arctic Biology University of Alaska Fairbanks P.O. Box 757000 Fairbanks AK 99775-0180 United States

(907) 474-7153 (907) 474-6967 rwruess@alaska.edu http://www.iab.uaf.edu/~roger_ruess/ Principal Investigator Michelle Cailin Mack Bonanza Creek LTER Lead Principal Investigator

Northern Arizona University: Ecosystem Science and Society Center (ECOSS) PO Box 5620 Flagstaff AZ 86011 United States

(928) 523-9415 Michelle.Mack@nau.edu https://macklab.nau.edu/ Principal Investigator NSF Grant numbers DEB-2224776, DEB-1636476, DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629 USDA Forest Service, Pacific Northwest Research Station (Agreement # RJVA-PNW-20-JV-11261932-018) summary_diatom_data.txt This data set contains diatom assemblages for 3 cores from the center, moat and surrounding burn (0, 12, and 30 m) of the BBC collapse scar. Three well-preserved cores were collected in March 2003 with a gasoline-powered permafrost corer. summary_diatom_data.txt 1 \n \n column \t http://www.lter.uaf.edu/php/download_data.php?f=/ascii/files/summary_diatom_data.txt Sample Name Sample Name Sample name used string Sample name used -9999 Missing Value Sample ID Sample ID Sample identification used by the Harden Lab at the USGS in Menlo Park string Sample identification used by the Harden Lab at the USGS in Menlo Park -9999 Missing Value Ecotone Ecotone Ecological type along the transect (bog, moat or burn) string Ecological type along the transect (bog, moat or burn) -9999 Missing Value Distance along transect (m) Distance along transect (m) Distance along the transect (0-30m) Integer Distance along the transect (0-30m) -9999 Missing Value Depth (cm) Depth (cm) Depth of sample in the soil profile (in cm) Integer centimeter real -9999 Missing Value NumberOfCategories NumberOfCategories Number of diatom categories in sample Integer Number of diatom categories in sample -9999 Missing Value Index of Categories Index of Categories Index of Categories, a mesure of the category eveness (num of categories in sample/total num of categories) integer Index of Categories, a mesure of the category eveness (num of categories in sample/total num of categories) -9999 Missing Value Simpson's Index Simpson's Index Simpson's Index, a measurement of the category richness and diversity (1-(Sum of ((num of valves per category/total num of valves)exp2))) integer Simpson's Index, a measurement of the category richness and diversity (1-(Sum of ((num of valves per category/total num of valves)exp2))) -9999 Missing Value Charcoal (bits/cm3) Charcoal (bits/cm3) The macroscopic specs of charcoal observed in the sample (in bits per cm3) double bits/cm3 real -9999 Missing Value Bulk Density (g/cm3) Bulk Density (g/cm3) The bulk density of the sample (in g per cm3) double gramsPerCubicCentimeter real -9999 Missing Value % Nitrogen % Nitrogen Percent nitrogen in the plant sample float percent real -9999 Missing Value % Carbon % Carbon Percent carbon in the plant sample float percent real -9999 Missing Value Carbon:Nitrogen Carbon:Nitrogen The ratio of the percent carbon to the percent nitrogen float dimensionless real -9999 Missing Value Mean Deuterium (%) Mean Deuterium (%) Mean deuterium (D or 2H reported per mil) float dimensionless real -9999 Missing Value Mean Oxygen 18 (%) Mean Oxygen 18 (%) Mean oxygen 18 (18O reported per mil) float dimensionless real -9999 Missing Value diatom_photos.zip This data set contains diatom assemblages for 3 cores from the center, moat and surrounding burn (0, 12, and 30 m) of the BBC collapse scar. Three well-preserved cores were collected in March 2003 with a gasoline-powered permafrost corer. diatom_photos.zip zip file http://www.lter.uaf.edu/php/download_data.php?f=/ascii/files/diatom_photos.zip zip file