uid=BNZ,o=lter,dc=ecoinformatics,dc=org all public read knb-lter-bnz.188.10 Isla Myers-Smith

405 CW Biological Sciences Building, Dept. Biological Sciences University of Alberta, , AB, T6G 2E9 Edmonton Aberta T6G 2E9 Canada

(780) 492-1295 myerssmi@ualberta.ca http://www.ualberta.ca/~myerssmi/home.html Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 jpdowning@alaska.edu http://www.lter.uaf.edu F.S. Stuart Chapin Professor of Ecology

Department of Biology and Wildlife; Institute of Arctic Biology: University of Alaska Fairbanks 193 Arctic Health P.O. Box 757000 Fairbanks AK 99775 United States

(907) 474-7922 (907) 474-6967 Associated investigator Jennifer W. Harden USGS, Adjunct Professor of Soil Science, University of Alaska Fairbanks

U.S. Geological Survey 345 Middlefield Road, MS 962 Menlo Park CA 94025 United States

(650) 329-4949 (650) 329-4920 Associated investigator A. David McGuire Assistant Professor of Landscape Ecology

Alaska Cooperative Fish and Wildlife Research Unit University of Alaska Fairbanks Irving I Building Fairbanks AK 99775 United States

(907) 474-6242 (907) 474-7872 Associated investigator 2005-11-12 This data set contains diatom assemblages for 3 cores from the center, moat and surrounding burn (0, 12, and 30 m) of the BBC collapse scar. Three well-preserved cores were collected in March 2003 with a gasoline-powered permafrost corer. Cores were stored frozen and sampled using a radial saw. The cores were sampled every two cm for macrofossil, diatom analysis and chemistry. Diatoms have been shown to be a useful indicator of peatland succession, local hydrology, and fire disturbance (Kienel et al., 1999, Moser et al., 2000, Rühland et al., 2000). Diatoms are more sensitive to changes in water levels than the commonly used peat indicators such as pollen and macrofossil analysis (Rühland et al., 2000). In this study, we employed diatom analysis to indicate shifts in water chemistry and to ascertain the response of this ecosystem to disturbance. To survey the diatom community, we processed three cores sampled every 2 cm by depth from the bog, moat and burn using methods described by the Paleoecological Environmental Assessment and Research Laboratory (PEARL) at Queen’s University and personal communications (Kathleen Rühland and John P. Smol). We digested the dried material, consisting of organic matter with varying amounts of sediment, in Kjeldahl digestion tubes in a heating block. We used 50:50 solution by molecular weight of concentrated H2SO4 and HNO3 and digested for 3 days at 95?C or until the disappearance of all organic matter. We diluted the resulting solution with deionized water to a neutral pH. We mounted the samples using the Pleurax high refractive index mounting medium (prepared by W. Dailey, University of Pennsylvania). To determine the prevalence of the different diatoms, we counted 400 valves (or for one sparse sample, four slides) for each sample. We identified samples according to Foged (1981), Krammer and Lange-Bertalot (1991) and personal communications with K. Rühland. We measured bulk density, %C and %N for all soil samples. We oven-dried at 50 - 65°C and ground all samples before analysis. We analyzed samples for %C and %N using a Carlo Erba EA1108 CHNS analyzer (CE Instruments, Milan, Italy) and a COSTECH ECS 4010 CHNS-O analyzer (Costech Analytical Technologies Inc., Valencia, CA,USA). Sample standard errors were ± 0.01% for nitrogen, ± 0.45% for carbon. To indicate fire events in the surrounding ecosystem, charcoal layers in the cores were quantified. We estimated charcoal by emptying dried samples of a known volume and depth (on mean 4.5 cm3) over a 10 cm x 10 cm grid and counting macroscopic charcoal fragments (greater than 0.05 mm in diameter) in each cm grid cell. moat diatoms fires macrofossil bog density carbon nitrogen soil samples charcoal Biogeochemistry

While metadata will be freely available to those requesting it, the data manager will assure that any restrictions on access to data sets in the database will be enforced. Data will not be released without proper permission first being obtained from the investigator who generated the data.

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http://www.lter.uaf.edu/data_detail.cfm?datafile_pkey=188 Site information unavailable -148.3334833 -148.3334833 64.6408 64.6408 2003-03-01 2003-03-29

Completed

Bonanza Creek LTER Data Manager

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 jpdowning@alaska.edu http://www.lter.uaf.edu Bonanza Creek LTER

Boreal Ecology Cooperative Research Unit University of Alaska Fairbanks P.O. Box 756780 Fairbanks AK 99775 USA

907-474-6364 907-474-6251 Bonanza Creek LTER

This data set contains diatom assemblages for 3 cores from the center, moat and surrounding burn (0, 12, and 30 m) of the BBC collapse scar and associated soil characteristics

Field methods: Three well-preserved cores were collected in March 2003 with a gasoline-powered permafrost corer. Lab methods: Cores were stored frozen and sampled using a radial saw. The cores were sampled every two cm for macrofossil, diatom analysis and chemistry. To survey the diatom community, we processed three cores sampled every 2 cm by depth from the bog, moat and burn using methods described by the Paleoecological Environmental Assessment and Research Laboratory (PEARL) at Queen’s University and personal communications (Kathleen Rühland and John P. Smol). We digested the dried material, consisting of organic matter with varying amounts of sediment, in Kjeldahl digestion tubes in a heating block. We used 50:50 solution by molecular weight of concentrated H2SO4 and HNO3 and digested for 3 days at 95?C or until the disappearance of all organic matter. We diluted the resulting solution with deionized water to a neutral pH. We mounted the samples using the Pleurax high refractive index mounting medium (prepared by W. Dailey, University of Pennsylvania). To determine the prevalence of the different diatoms, we counted 400 valves (or for one sparse sample, four slides) for each sample. We identified samples according to Foged (1981), Krammer and Lange-Bertalot (1991) and personal communications with K. Rühland. We measured bulk density, %C and %N for all soil samples. We oven-dried at 50 - 65°C and ground all samples before analysis. We analyzed samples for %C and %N using a Carlo Erba EA1108 CHNS analyzer (CE Instruments, Milan, Italy) and a COSTECH ECS 4010 CHNS-O analyzer (Costech Analytical Technologies Inc., Valencia, CA,USA). Sample standard errors were ± 0.01% for nitrogen, ± 0.45% for carbon. To indicate fire events in the surrounding ecosystem, charcoal layers in the cores were quantified. We estimated charcoal by emptying dried samples of a known volume and depth (on mean 4.5 cm3) over a 10 cm x 10 cm grid and counting macroscopic charcoal fragments (greater than 0.05 mm in diameter) in each cm grid cell.

geoReference 2003-03-01 2003-03-29

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raw_diatom_data.txt raw_diatom_data.txt 1 \n \n column \t http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-bnz..&urlTail=/ascii/files/raw_diatom_data.txt Sample Name Sample Name Sample name used string Sample name used -9999 Missing Value Sample ID Sample ID Sample identification used by the Harden Lab at the USGS in Menlo Park string Sample identification used by the Harden Lab at the USGS in Menlo Park -9999 Missing Value Ecotone Ecotone Ecological type along the transect (bog, moat or burn) string Ecological type along the transect (bog, moat or burn) -9999 Missing Value Distance along transect (m) Distance along transect (m) Distance along the transect (0-30m) Integer Distance along the transect (0-30m) -9999 Missing Value Depth (cm) Depth (cm) Depth of sample in the soil profile (in cm) Integer centimeter real -9999 Missing Value Category # Category # Number of diatom categories in sample Integer Number of diatom categories in sample -9999 Missing Value Genus/species Genus/species The name of the Genus, Species or category of diatom string The name of the Genus, Species or category of diatom -9999 Missing Value Size (um) Size (um) The length of the diatom valve (micrometers) Integer The length of the diatom valve (micrometers) -9999 Missing Value Slide Slide The slide counted (slide A, slide B, slide C, or slide D) string The slide counted (slide A, slide B, slide C, or slide D) -9999 Missing Value Diatom Assemblage (proportion of total valves) Diatom Assemblage (proportion of total valves) The proportion of each category of diatoms of the total number of valves counted (400 valves) float percent real -9999 Missing Value Charcoal (bits/cm3) Charcoal (bits/cm3) The macroscopic specs of charcoal observed in the sample (in bits per cm3) double bits/cm3 real -9999 Missing Value diatom_photos.zip diatom_photos.zip 1 \n \n column \t http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-bnz..&urlTail=/ascii/files/raw_diatom_data.txt Sample Name Sample Name Sample name used string Sample name used -9999 Missing Value Sample ID Sample ID Sample identification used by the Harden Lab at the USGS in Menlo Park string Sample identification used by the Harden Lab at the USGS in Menlo Park -9999 Missing Value Ecotone Ecotone Ecological type along the transect (bog, moat or burn) string Ecological type along the transect (bog, moat or burn) -9999 Missing Value Distance along transect (m) Distance along transect (m) Distance along the transect (0-30m) Integer Distance along the transect (0-30m) -9999 Missing Value Depth (cm) Depth (cm) Depth of sample in the soil profile (in cm) Integer centimeter real -9999 Missing Value Category # Category # Number of diatom categories in sample Integer Number of diatom categories in sample -9999 Missing Value Genus/species Genus/species The name of the Genus, Species or category of diatom string The name of the Genus, Species or category of diatom -9999 Missing Value Size (um) Size (um) The length of the diatom valve (micrometers) Integer The length of the diatom valve (micrometers) -9999 Missing Value Slide Slide The slide counted (slide A, slide B, slide C, or slide D) string The slide counted (slide A, slide B, slide C, or slide D) -9999 Missing Value Diatom Assemblage (proportion of total valves) Diatom Assemblage (proportion of total valves) The proportion of each category of diatoms of the total number of valves counted (400 valves) float percent real -9999 Missing Value Charcoal (bits/cm3) Charcoal (bits/cm3) The macroscopic specs of charcoal observed in the sample (in bits per cm3) double bits/cm3 real -9999 Missing Value