| Title: |
N-fixation rate and leaf N content in two species of Alnus and their relationship to diversity of symbiotic Frankia
|
| Contacts: |
Ruess, Roger
Anderson, Mike
Taylor, Lee
|
| Abstract: |
This study investigated patterns of nitrogen (N) fixation rates, leaf N content, and geographic diversity in the N-fixing bacterium Frankia occurring in symbiosis with Alnus incana ssp. tenuifolia and A. viridis ssp. fruticosa in early and late successional habitats on the Tanana river floodplain and surrounding uplands in the Bonanza Creek Experimental Forest. Frankia diversity was estimated via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the nifD-K spacer region, a non-coding region in the nitrogenase-encoding operon. Specific N-fixation rate was measured with a 15N2 uptake assay and leaf N content via mass spectrometry. Additional parameters measured were soil temperature and moisture, leaf del 15N, and specific leaf area.
|
| Keywords: |
actinorhizal, Alder, alnus, frankia, nitrogen fixer, |
| Categories: |
Biogeochemistry
Primary Production
|
| Study Period: |
Jun 19, 2002 to Sep 1, 2002
|
| Bounding Box: |
West longitude: -148.324901°
North latitude: 64.7666796°
East longitude: -148.1435205°
South latitude: 64.6719571°
|
| Site References: |
FP1A -- FP1A is located on a low early successional terrace (1.8 - 2 m above winter low river level) adjacent to the Tanana River. Vegetation establishment occurred in 1982 to 1983. Significant bank erosion has occurred since the establishment of the study area, resulting in loss of some study plots.
FP1B -- FP1B is located on a low early successional terrace adjacent to the Tanana River.
FP1C -- FP1C is located on a low early successional terrace adjacent to the Tanana River.
FP4A -- Mature white spruce
FP4B -- Mature white spruce
FP4C -- Mature white spruce
UP1A -- UP1A was established to follow the development of vegetation following the burning of a well developed 200 year old white spruce stand on a south facing slope. The vegetation has progressed from an herbaceous community following the fire to an open hardwood stand.
UP1B -- Young Paper Birch/Shrub/Herbaceous Stand
UP1C -- Young Aspen Forest
UP3A -- Mature white spruce spruce stand on a south-facing slope
UP3B -- Closed White Spruce Forest
UP3C -- Closed White Spruce Forest
|
| Data Columns: |
| Column |
Name |
Units |
Type |
| 1 |
Land |
none |
string |
| 2 |
Stage |
none |
string |
| 3 |
Rep |
none |
integer |
| 4 |
Sampper |
none |
integer |
| 5 |
Species |
none |
string |
| 6 |
PLNO |
none |
integer |
| 7 |
JULDAY |
none |
integer |
| 8 |
1CM |
Celsius |
float |
| 9 |
5CM |
Celsius |
float |
| 10 |
PERH2O |
percent |
float |
| 11 |
SNF |
gram |
float |
| 12 |
RF |
none |
float |
| 13 |
SLA |
gramsPerSquareMeter |
float |
| 14 |
LEAFPERN |
percent |
float |
| 15 |
DEL |
|
float |
|
| Data File(s): |
227_2061_n_fixation_leafn_and_frankia_diversity.txt
|
| Supplemental Files: |
|
| Metadata Formats: |
|
| Public Release: |
Nov 17, 2005
|
| Publications: |
Anderson, M.D., R.W. Ruess, D.D. Uliassi and J.S. Mitchell. 2004. Estimating N2 fixation in two species of Alnus in interior Alaska using acetylene reduction and 15N2 uptake. Ecoscience 11:102-112.
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The Bonanza Creek LTER, including this website, is supported by the
National Science Foundation through awards
DEB-0620579,
DEB-0423442,
DEB-0080609,
DEB-9810217,
DEB-9211769,
DEB-8702629
and by the USDA Forest Service, Pacific Northwest Research Station through agreement number RJVA-PNW-01-JV-11261952-231.
Any opinions, findings, conclusions, or recommendations expressed in the
material are those of the author(s) and do not necessarily reflect
the views of the supporting agencies or the program as a whole.
© Bonanza Creek LTER, 2009.
Last modified 23-Nov-09
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This site is a member of the LTER Network.
Disclaimer
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